Volume 4 Issue Spl-3-ADPCIAD Article 7

Sero monitoring of anti rabies vaccinal antibodies to pep in animals by RFFIT - a case study in Karnataka

Sunilkumar KM2; Isloor S1,*; Ansar Kamran C2; Santosh AK1; Ramesh PT2; Sharada R3; Yathiraj S2; Rathnamma D1; Suryanarayana T2; Patil SS4; Abhinandan Patil4;

1KVAFSU-CVA-Crucell Rabies Diagnostic Laboratory, Dept. of Microbiology,
2Dept. of Medicine, Veterinary College, KVAFSU, Hebbal, Bengaluru-560024, Karnataka
3Dept. of Microbiology, Veterinary college, KVAFSU, Hassan, Karnataka
4Veterinary Hospital, Department of Animal Husbandry and Vety. Services, Govt. of Karnataka, Gangavathi-, Koppal, Karnataka

Keywords: Rabies, Post exposure prophylaxis, Neutralizing antibodies, Animals and RFFIT

Page No: S103 - S107

Received - August 25, 2016; Revision - October 14, 2016; Accepted - October 27, 2016
Available Online - October 30, 2016


A dog suspected to be affected with rabies attacked 31 animals in Kesarahatti village of Gangavathi taluk, Koppal District in Karnataka on 14th June 2015. Of these, 26 animals (14 cattle, 5 buffaloes and 7 dogs) were given the first aid followed by post exposure anti rabies vaccine at 1 (15th June 2015), 3, 7, 14 and 28 days interval whereas the remaining 5 animals (2 cattle and 3 buffaloes) reportedly died of suspected (based on clinical manifestation) rabies after 8 days of dog bite episode. These 5 animals were not provided first aid and post exposure prophylactic (PEP) vaccination as their exposure to the rabid dog bite went unnoticed. Further, the serum samples could be collected from only 21 out of 26 vaccinated animals on 28th and 90th day post vaccination. These serum samples were tested by Rapid Fluorescent Focus Inhibition Test (RFFIT) and the titre of neutralizing antibodies ranged from 1-8 IU/ml from the serum collected on 28th day post vaccination indicating protection in all the vaccinated animals. But the titre ranged from 0.5-4 IU/ml in 18 animals and less than 0.5 IU/ml in three animals (2 cows and a dog) on 90th day post vaccination. The protective level of neutralizing antibody titre on day 28 post vaccination and non-development of disease suggests that the PEP vaccination could be effective in livestock avoiding euthanasia and culling of animals.

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