Volume 4 Issue Spl-3-ADPCIAD Article 6

Effect of melatonin on mobility and velocity parameters of mithun (Bos frontalis) semen preserved in liquid state (5OC)


Perumal P1,*; Chang S1; Khate K1; Sangma CTR2; Savino N2;


1ICAR- National Research Centre on Mithun, Jharnapani, Nagaland- 797 106, India
2NU-School of Agricultural Science and Rural Development, Medziphema, Nagaland- 797 106, India



Keywords: Melatonin, Mithun, Computer assisted sperm analysis and Liquid semen preservation

Page No: S95 - S102


Received - August 08, 2016; Revision - October 25, 2016; Accepted - October 26, 2016
Available Online - October 30, 2016



Abstract


Present study was conducted to evaluate the effect of melatonin (MEL) on velocity and motility of mithun semen by computer assisted sperm analyser. Semen ejaculates (n=25) were collected from matured mithun bulls (n=10) using trans-rectal massage method and were divided and grouped into six aliquots equally, extended with the standard Tris Egg Yolk Citrate semen diluent. Six groups for various treatments were prepare, these group were control - semen without MEL (Gr 1), 1mM (Gr 2), 2mM (Gr 3), 3Mm (Gr 4), 4mM (Gr 5:) and 5mM (Gr 6). Various parameter such as total motility, forward progressive motility, straight line velocity, curvilinear velocity, average path velocity, wobble, linearity, straightness, beat/cross frequency, amplitude of lateral head displacement and velocity of rapid, medium, slow and static were measured for 0-30h at 6 hrs interval at 5oC. The result observed that these mobility and velocity parameters were varied significantly (p<0.05) among the experimental periods and among the experimental groups. Further, MEL at 3 mM has significant (p<0.05) improvement in the mobility and velocity parameters than MEL at 1, 2, 4or 5 mM stored in in- vitro for up to 30 h of incubation. It was concluded that MEL 3 mM treated sperm has increased functional sperm structures faster to move and forward direction, probably improves the fertilization rate.

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