Volume 4 Issue Spl-3-ADPCIAD Article 2

Diagnosis of animal rabies: comparison of direct fluorescent antibody test (DFAT), reverse transcriptase -PCR and real-time PCR


Manjunathareddy GB1,*, Sumana K1, Yogisharadhya R1, Susan Cherian2, Prajapati A1, Patil SS1, Balamurugan V1, Singh KP2, Singh R2 and Rahman H1


1ICAR-National Institute of Veterinary Epidemiology and Disease Inforamtics (NIVEDI), Yelahanka, Bengaluru, Karnataka 560064. India
2Division of Veterinary Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, U.P 243122. India



Keywords: Animal, Diagnosis, Rabies, RT-PCR and RT-qPCR

Page No: S69 - S74


Received - August 01, 2016; Revision - October 08, 2016; Accepted - October 25, 2016
Available Online - October 30, 2016



Abstract


Rabies is endemic in most parts of India, with the exception of Andaman and Nicobar, Lakshadweep islands and to some extent Nagaland. For prevention and control it is essential to rapidly and precisely diagnose rabies. In this study, we used three diagnostic methods, direct fluorescent antibody test (dFAT), reverse transcriptase polymerase chain reaction (RT-PCR) and real time reverse transcriptase polymerase chain reaction (RT-qPCR) to detect the rabies virus in suspected animal brains. Out of the 80 animal brain samples tested, 64 (80%) were positive for rabies according to the RT-qPCR. Compared to the RT-qPCR, the sensitivities of dFAT and RT-PCR were 95.31% and 96.88%, respectively. The specificities of dFAT and RT-PCR were on far with qRT-PCR. Even though the dFAT findings did not completely coincide with results obtained from RT-PCR and RT-qPCR, dFAT appears to be a fast and reliable assay that can be used to analyze fresh brain samples. But in countries like India where temperature reaches 50�C during summer and lack of diagnostic facilities and trained personnel to carry out the dFAT at field level, the suspected samples will be usually sent to National/Regional Disease Diagnostic Laboratory /State veterinary or agricultural universities for rabies diagnosis. In summary the molecular methods RT-PCR and RT-qPCR can serve as quick and rapid diagnostic methods for animal rabies in India.

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