Volume 5 Issue 6 Article 22

Assessment of agriculturally important metabolites from the entomopathogenic bacterium, Photorhabdus temperata M1021

 

Ihsan Ullah1*, Yasir Anwar1, Khalid M S Al-Ghamdi1, Ahmad Firoz1, Jae-Ho Shin2

 

1Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
2School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea

 

Keywords: [Plant microbe interaction, Photorhabdus temperate, Plant growth promotion, Insecticidal activity, Galleria mellonella]

Page No:907 – 914

 

Received – December 01, 2017; Revision – December 20, 2017; Accepted – December 25, 2017
Available Online – December 27, 2017

 

Abstract

 

Photorhabdus temperata M1021 is an entomopathogenic enterobacterium, producing an array of metabolites in the culture extract. Photorhabdus spp. produced various classes of toxic proteins and secondary metabolites, which generate mortality in a wide range of insects. Aim of present study was to assess the effects these metabolites towards the insect and plants. Intra-hemocoel injections of whole cell and soluble extract caused 100% mortality in Galleria mellonella larvae within 48 h as compare to control. However, the culture filtrate (CF) took 72 h to kill 100 % larvae. In addition, there was no significant (P<0.05) decrease in the rate of larval mortality, injected with CF, treated at 70°C for 30 min as well as with 50 mg/l proteinase-K. Gas chromatography mass spectrometry analysis revealed 23 compounds, among which “trans-Cinnamic acid” and “indole” were the most important for the insecticidal activities and plant growth promotion. The auxin contents quantified through Salkowski’s assay were 28 µg/ml to 30 µg/ml, insignificantly varied the by the addition of L-tryptophan ranged from 0 g/l to 0.5 g/l. The optimum pH for the auxin production was determined to be pH 7.0. The CF treatment increases significantly (P<0.05) plant growth that attributes e.g. total plant length, chlorophyll content and biomass of both Dongjin-beyo and Waito-Crice plants, in a comparison with control. The findings of this study suggest that the P. temperata M1021 could be an ideal candidate, to be exploited in agriculture as biocontrol agent.

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