Volume 5 Issue 2 Article 15

Studies on endophytic Burkholderia sp. from sugarcane and its screening for plant growth promoting potential


R. Arthee*; P. Marimuthu;


Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore-03



Keywords: Burkholderia, MALDI-TOF MS, Plant growth promotion, Phylogeny

Page No: 242 - 257


Received - March 31, 2017; Revision - April 27, 2017; Accepted - May 01, 2017
Available Online - May 12, 2017



Abstract


The occurrence of Burkholderia genus as plant endophyte indigenous to locally cultivated sugarcane crop was studied. Totally 22 endophytic isolates were obtained from TB-T medium plates of which 10 were found to be putatively endophytic in sugarcane. The morphological, biochemical and MALDI-TOF MS analysis were done and 5 endophytic Burkholderia sp. isolates were identified. Among them, the isolate Burkholderia sp. ES4 shown maximum; diazotrophic activity (total nitrogen: 11.9 mg g-1 of malate; nitrogenase activity: 578.3 n mol C2H4 released h-1 mg-1protein), mineral solubilization activity (10.2mm, 8.2mm and 3.2 mm solublization zone of P, ZnO and CaCO3 respectively; available phosphorous: 0.72mg ml-1), antagonistic activity (54.56 percent inhibition of Colletotrichum falcatum mycelium) and phytohormone production (IAA (16.09 μg ml-1); GA (10.54 μg ml-1)), thereby revealing its plant growth promoting potential. The 16S r RNA gene sequence of the isolate Burkholderia sp. ES4 was sequenced and its phylogenetic analysis shown that the isolate forms a monophyletic subclade within the plant-beneficial-environment' (PBE) Burkholderia group. Thus a potent native plant endophytic strain, Burkholderia sp. ES4 that could be developed as biofertilizer inoculum for sugarcane crop, was obtained in this study.

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