Volume 4 Issue VIS Article 2

Cloning and expression pattern analysis of MmPOD12 gene in mulberry under abiotic stresses


Rongfang Li; Dandan Chen; Dominic Kwame Kotoka; Renjie Sun; Yuanliang Deng; Feng Li; Jiao Qian; Rongjun fang; Long Li ; Weiguo Zhao*;


School of Biology and Technology, Jiangsu University of Science and Technology, Sibaidu, Zhenjiang, Jiangsu212018,PR China



Keywords: Mulberry, MmPOD12 gene, Gene cloning and expression and Abiotic stress

Page No: 698 - 705


Received - August 11, 2016; Revision - September 09, 2016; Accepted - December 16, 2016
Available Online - December 29, 2016



Abstract


A full-length cDNA denominated as MmPOD12 for peroxidase in mulberry(Morus alba), an enzyme involved in the respiration, photosynthesis, and the oxidation of auxin, was cloned from 'Yu71-1' a variety of mulberry using a rapid amplification of terminal (RACE) approach. The full cDNA of MmPOD12 has1482 base pairs (bp) in length with an open reading frame (ORF) 1050 bp encoding a protein of 349 amino acids residues with a predicted molecular weight of 53.92 kDa and an isoelectric point of 9.35. Sequence analysis revealed that MmPOD12 shares homology with the Morus notabili (M. notabilis C.K.Schn) and has closely related to green plum, strawberry and pear. The expression patterns of MmPOD12 treated with drought, salt and hormones stresses were examined using real-time quantitative PCR (RT-qPCR). These experiments caused significant up-regulation of the expression of MmPOD12 under drought and salt stress. The highest expression level of MmPOD12 appeared at 2d for salt stress, and 7d for drought stress, while a significant fluctuation of MmPOD12 expression was detected after ABA and SA stresses. These findings provide a basis for future functional analyses of MmPOD12 gene in Mulberry.

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